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Directly Bound Deuterons Increase X-Nuclei Hyperpolarization using Dynamic Nuclear Polarization |
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| Authors: | Catriona H E Rooney Dr Ayelet Gamliel David Shaul Prof Damian J Tyler Dr James T Grist Prof Rachel Katz-Brull |
| Institution: | 1. Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK
Equal contribution;2. Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, 9112011 Israel The Wohl Institute for Translational Medicine, Hadassah Medical Organization, Jerusalem, Israel Equal contribution;3. Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, 9112011 Israel The Wohl Institute for Translational Medicine, Hadassah Medical Organization, Jerusalem, Israel;4. Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK Oxford Centre for Clinical Magnetic Resonance Research, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK;5. Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK Oxford Centre for Clinical Magnetic Resonance Research, Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford, UK Department of Radiology, Oxford University Hospitals, Oxford, UK Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy Equal contribution;6. Department of Radiology, Hadassah Medical Organization and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, 9112011 Israel |
| Abstract: | Deuterated 13C sites in sugars (D-glucose and 2-deoxy-D-glucose) showed 6.3-to-17.5-fold higher solid-state dynamic nuclear polarization (DNP) levels than their respective protonated sites at 3.35T. This effect was found to be unrelated to the protonation of the bath. Deuterated 15N in sites bound to exchangeable protons (15N2]urea) showed a 1.3-fold higher polarization than their respective protonated sites at the same magnetic field. This relatively smaller effect was attributed to incomplete deuteration of the 15N sites due to the solvent mixture. For a 15N site that is not bound to protons or deuterons (15N]nitrate), deuteration of the bath did not affect the polarization level. These findings suggest a phenomenon related to DNP of X-nuclei directly bound to deuteron(s) as opposed to proton(s). It appears that direct binding to deuterons increases the solid-state DNP polarization level of X-nuclei which are otherwise bound to protons. |
| Keywords: | D-glucose 2-deoxy-D-glucose solid-state polarization urea sodium nitrate |