Composite Mesoporous Silica Nanoparticles with Dual-color Afterglow for Cross-correlation-based Living Cell Imaging |
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Authors: | Mingqi Sun Jiaqi Meng Weier Bao Dr Ming Liu Xiaojuan Li Zicheng Wang Zhecheng Ma Prof?Dr Xuefei Wang Prof?Dr Zhiyuan Tian |
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Institution: | School of Chemical Sciences, University of Chinese Academy of Sciences (UCAS), Beijing, 100049 China |
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Abstract: | Room temperature phosphorescence (RTP) materials are characterized with emission after removing the excitation source. Such long-lived emission feature possesses great potential in biological fluorescence imaging because it enables a way regarding temporal dimension for separating the interference of autofluorescence and common noises typically encountered in conventional fluorescence imaging. Herein, we constructed a new type of mesoporous silica nanoparticles (MSNs)-based composite nanoparticles (NPs) with dual-color long-lived emission, namely millisecond-level green phosphorescence and sub-millisecond-level delayed red fluorescence by encapsulating a typical RTP dye and Rhodamine dye in the cavities of the MSNs with the former acting as energy donor (D) while the latter as acceptor (A). Benefiting from the close D-A proximity, energy match between the donor and the acceptor and the optimized D/A ratio in the composite NPs, efficient triplet-to-singlet Förster resonance energy transfer (TS-FRET) in the NPs occurred upon exciting the donor, which enabled dual-color long-lived emission. The preliminary results of dual-color correlation imaging of live cells based on such emission feature unequivocally verified the unique ability of such NPs for distinguishing the false positive generated by common emitters with single-color emission feature. |
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Keywords: | Room temperature phosphorescence triplet-to-singlet Förster resonance energy transfer (TS-FRET) cross-correlation imaging false positive |
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