Monitoring of vesicular exocytosis from single cells using micrometer and nanometer-sized electrochemical sensors |
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Authors: | Wei Wang Shu-Hui Zhang Lin-Mei Li Zong-Li Wang Jie-Ke Cheng Wei-Hua Huang |
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Institution: | (1) College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China |
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Abstract: | Communication between cells by release of specific chemical messengers via exocytosis plays crucial roles in biological process.
Electrochemical detection based on ultramicroelectrodes (UMEs) has become one of the most powerful techniques in real-time
monitoring of an extremely small number of released molecules during very short time scales, owing to its intrinsic advantages
such as fast response, excellent sensitivity, and high spatiotemporal resolution. Great successes have been achieved in the
use of UME methods to obtain quantitative and kinetic information about released chemical messengers and to reveal the molecular
mechanism in vesicular exocytosis. In this paper, we review recent developments in monitoring exocytosis by use of UMEs-electrochemical-based
techniques including electrochemical detection using micrometer and nanometer-sized sensors, scanning electrochemical microscopy
(SECM), and UMEs implemented in lab-on-a-chip (LOC) microsystems. These advances are of great significance in obtaining a
better understanding of vesicular exocytosis and chemical communications between cells, and will facilitate developments in
many fields, including analytical chemistry, biological science, and medicine. Furthermore, future developments in electrochemical
probing of exocytosis are also proposed.
Figure In this paper, we review recent developments in monitoring the exocytosis by use of UMEs-electrochemical-based techniques
including electrochemical detection using micrometer and nanometer-sized sensors, Scanning Electrochemical Microscopy (SECM)
and UMEs implemented in lab-on-a-chip (LOC) microsystems. These advances are of great significance in obtaining a better understanding
of vesicular exocytosis and chemical communications between cells, and will facilitate developments in many fields including
analytical chemistry, biological science and medicine. Furthermore, future developments in electrochemical probing of exocytosis
are proposed.
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Keywords: | Exocytosis Micrometer and nanometer-sized electrochemical sensors Scanning electrochemical microscopy Microelectrode arrays Lab-on-a-chip Review |
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