幼鼠小肠缺血-再灌注肠组织的形态学观察

目的:观察幼鼠小肠缺血-再灌注(ischemia/reperfusion,I/R)肠组织形态学变化。方法:将32只4周龄(Sprague Dawley,SD)大鼠随机分成2组即假手术组(Sham组)和缺血-再灌注组(I/R组),每组各16只。Sham组仅暴露腹腔脏器,不作任何干预;I/R组经阻断肠系膜上动脉60 min后恢复血供,于再灌注30、60、90、120 min每组动物各处死4只取小肠组织进行组织学Chiu’s评分,结果进行单因素方差分析和组间t检验。结果:Sham组肠组织形态正常;I/R组小肠组织发生了明显的缺血和炎症反应并随再灌注时间延长而加重,各观察时间点组织学评分在组间比较差异均有统计学意义,各值在I/R组高于Sham组(P<0.05)。结论:幼鼠肠I/R导致肠组织病理损伤并随再灌注时间延长而加重。

Histological Study of Intestine with Ischemia/Reperfusion Injury in Young Rats

Objective: To explore the intestinal histological changes of intestinal ischemic/reperfusion(I/R) injury in young rats.Methods: 32 Sprague Dawley(SD) rats at 4 weeks old were divided into 2 groups with 16 in each group randomly,and there were sham operated group as a control and I/R group.The abdominal cavity were exposed in all rats but not any intervene in sham group,the superior mesenteric artery were occluded in I/R group for 60 min and followed by 30 min,60 min,90 min and 120 min for reperfusion and 4 rats were harvested at each time point.The intestine specimens were obtained for pathological study and determination of histopathologic score.The data were analyzed statistically.Results: There were pathological changes of ischemia and inflammatory obviously and getting severer with the reperfusion time prolong in all rats but in the sham group.There was statistical significance as a result of the Chiu’s score between two groups in every time point(P<0.05).The scores in I/R group were higher than that in sham group(P<0.05).The score was highest at 90min among four time points in each I/R group.Conclusion: The pathological changes of intestinal injury caused by I/R were getting severer with reperfusion prolong.