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Neutron-Encoded Protein Quantification by Peptide Carbamylation |
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| Authors: | Arne Ulbrich Anna E Merrill Alexander S Hebert Michael S Westphall Mark P Keller Alan D Attie Joshua J Coon |
| Institution: | 1. Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA 3. Genome Center of Wisconsin, University of Wisconsin, Madison, WI, 53706, USA 2. Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI, 53706, USA 4. Department of Biochemistry, University of Wisconsin, Madison, WI, 53706, USA |
| Abstract: | We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet. ? |
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