The Impact of Long-Term In Vitro Expansion on the Senescence-Associated Markers of Human Adipose-Derived Stem Cells |
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| Authors: | Wan Kamarul Zaman Wan Safwani Suzana Makpol Somasundaram Sathapan Kien Hui Chua |
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| Institution: | (1) Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;(2) Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;(3) Subang Jaya Medical Centre, Selangor, Malaysia;(4) Department of Physiology, Faculty of Medicine, Jalan Raja Muda Abdul Aziz, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia |
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| Abstract: | Human adipose-derived stem cells (ASCs) have generated a great deal of excitement in regenerative medicine. However, their safety and efficacy issue remain a major concern especially after long-term in vitro expansion. The aim of this study was to investigate the fundamental changes of ASCs in long-term culture by studying the morphological feature, growth kinetic, surface marker expressions, expression level of the senescence-associated genes, cell cycle distribution and ß-galactosidase activity. Human ASCs were harvested from lipoaspirate obtained from 6 patients. All the parameters mentioned above were measured at P5, P10, P15 and P20. Data were subjected to one-way analysis of variance with a Tukey post hoc test to determine significance difference (P?
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