Concentration of sodium ion as the determining factor for the association of dansyl amino acids with the teicoplanin molecule in reversed-phase liquid chromatography |
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Authors: | Ismaili Lhassane Truong Tong Than André Claire Thomassin Mireille Mozer Jean-Louis Robert Jean-Francois Xicluna Alain Refouvelet Bernard Millet Joelle Nicod Laurence Guillaume Yves-Claude |
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Institution: | Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Equipe des Sciences Séparatives et Biopharmaceutiques (2SB) Place St-Jacques, 25030 Besan?on Cedex, France. |
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Abstract: | The mechanism of the binding of D,L dansyl amino acids to teicoplanin was investigated. Na+ was used as an indicator of the interactions between the solutes and teicoplanin. The number (n) of sodium ions, Na+, excluded from the solute-teicoplanin interface when analyte transfer occurred was determined. A thermodynamic study and enthalpy-entropy compensation were performed to further explore the interaction mechanism. From these results, it was shown that teicoplanin was balanced between 2 conformational states characterized by distinct enantioselective properties. This approach indicates that liquid chromatography (LC) is a useful tool to extract physicochemical and molecular information from retention data. Thus, LC can be used as a complementary technique with the conventional techniques of molecular interaction analysis. |
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