转溶菌酶基因籼稻在湖南地区的抗稻瘟病研究

以转溶菌酶基因粳稻中花9号ZH9(R)]与几个湖南地区目前生产上有广泛应用价值但感病的籼型杂交稻亲本9311、MH63、288、E32和PA64s进行一次杂交和多次回交转育,从各转育世代中选育出优良的抗病株系,进而配制新的杂交稻组合,以期获得优良杂交稻亲本新品系。试验结果表明,各转育后代发病情况均明显轻于对应籼稻亲本;在这5个品种中,MH63要优于其它几个亲本,其转育后代抗性和农艺性状也相对优良,且已基本趋于稳定;田间筛选到的353份优良单株的DNA样中检测到269个阳性样,阳性检出率达76.20%,对应田间的抗性表现,吻合率达94.05%。

Investigation on Resistance of Indica Rices with Lysozyme Gene against Rice Blast in Hunan Area

The article deals with researches on obtaining new strains of highly resistance to create new hybrid rice with fine quality and strong resistance to rice blast germ through backcross between the lysozyme gene donor-transformed ZH-9(R) and general types 9311, MH63, 288, E32 and PA64s in Hunan. The results are as follow: Hybrid offspring were notably more resistant to rice blast than their parents. In the five parent indica rices, MH63 turned out to be superior to the others for that its transferring generations displayed well and stable in resistance and agronomic characters; Positive plants of PCR are 269 from 353 that screening from all of transferring generations, which is 76.20%; Contrast to resistance identification result, the ration of likeness reaches 94.05%.