Reversible 5′-end biotinylation and affinity purification of synthetic RNA |
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| Authors: | Shiyue Fang |
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| Affiliation: | Department of Medicinal Chemistry and Molecular Pharmacology, 201 S University Street, Purdue University, West Lafayette, IN 47907, USA Walther Cancer Institute, Indianapolis, IN 46208, USA |
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| Abstract: | A reversible biotinylation phosphoramidite was synthesized and incorporated onto the 5′-end of an oligoribonucleotide on a solid phase synthesizer. After cleavage and deprotection, the crude synthetic oligomer mixture was incubated with NeutrAvidin® coated microspheres, and the failure sequences removed by washing with a buffer followed by treating the microspheres with tetrabutylammonium fluoride to give a high quality unmodified full-length oligoribonucleotide. |
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| Keywords: | Oligoribonucleotide RNA Solid phase synthesis Reversible biotinylation Phosphoramidite Avidin Affinity purification Diisopropylsilylacetal linker |
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