Purification and dual-column HPLC determination of carboxylic acids in tissues,phloem and xylem saps of soybean plants |
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| Authors: | B Touraine S Astruc |
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| Institution: | (1) Laboratoire de Biochimie et Physiologie Végétales, école Nationale Supérieure d'Agronomie de Montpellier/Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique (Unité de Recherche Associée 573), Placa Viala, F-34060 Montpellier Cedex 01, France |
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| Abstract: | Summary A method for the determination of carboxylic acids in phloem sap, xylem sap and tissue extracts of soybean plants is reported.
An easy and rapid procedure for the purification of the samples has been developed. Carboxylic acids were determined by isocratic
HPLC. The separation of the first-eluting carboxylic acids and the unretained solutes have been improved by connecting an
ion-exclusion column to the reversed-phase column. This chromatographic method also improved the separation of some carboxylic
acids. The optimized method was applied to the determination of the carboxylic acids transported in xylem and phloem saps
of soybean plants, or accumulated in their root tissues. |
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| Keywords: | Column liquid chromatography Reversed-phase and ion-exclusion columns in series Carboxylic acids Sample purification Phloem and xylem saps |
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