高效液相色谱法同时测定化妆品中的9种水溶性着色剂

建立了用高效液相色谱-二极管阵列检测器(HPLC-DAD)同时检测化妆品中9种水溶性着色剂（溶剂绿7、食品黄3、食品红17、酸性黄1、酸性红33、食品红4、食品红1、橙黄I、酸性橙7）的检测方法。不同种类的化妆品采用不同的样品前处理方法提取后,用Diamonsil C18色谱柱分离,以乙腈-磷酸二氢钾缓冲溶液(pH 6)为流动相进行梯度洗脱,检测波长为240 nm,15 min内可对9种目标物同时进行检测,且各化合物都达到基线分离。经测定,该方法的平均回收率(n = 9)为85.33%～100.2%,相对标准偏差(RSD)为3.68%～8.20%,检出限为0.01～0.1 mg/L。方法简单、快速,能有效地提取、分离和测定化妆品中9种水溶性着色剂。将该方法用于实际化妆品的检测,结果令人满意。

Simultaneous determination of 9 water-soluble colorants in cosmetics by high performance liquid chromatography

An analytical method based on high performance liquid chromatography with diode array detector (HPLC-DAD) has been established for the simultaneous determination of 9 wa-ter-soluble colorants (Solvent Green 7, Food Yellow 3, Food Red 17, Acid Yellow 1, Acid Red 33, Food Red 4, Food Red 1, Orange I , Acid Orange 7) in cosmetics. The different kinds of samples were treated with different preparation methods. The obtained liquid samples were an-alyzed by HPLC using a Diamonsil C18 column (250 mm ×4. 6 mm, 5 μm). Acetonitrile-potas-sium dihydrogen phosphate (KH_2PO_4) buffer solution (pH 6) was used as the mobile phase for gradient elution. The 9 water-soluble colorants were well separated within 15 min. The average recoveries (n =9) were from 85. 33% to 100. 2% with the relative standard deviations (RSDs) between 3. 68% and 8. 20%. The limits of detection (LOD) were in the range of 0. 01 - 0. 1 mg/L. The method is simple, rapid, accurate and suitable for the determination of 9 water-sol-uble colorants in cosmetics.