Analytical fractionation of trace metals in human serum by means of a chelating ion-exchanger

Conclusions Both, in labile form bound trace metals as well as ionic ones in human serum can selectively be separated on the heavy-metal chelating cellulose HYPHAN under physiological conditions. From the separation kinetics, detailed informations on the binding stability of metal-proteins e.g., Cu(II), Fe(III), Zn(II)] result. Owing to low blanks, the separation procedure can also be applied to the fractionation of nanogram amounts of other metals e.g., Al(III), Be(II), Mn(II), Ni(II), Pb(II)] in serum. Furthermore, the coupling with a protein fractionation method (e.g., gelchromatography, isotachophoresis) allows to investigate the binding stability of isolated metal-proteins.

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Analytische Fraktionierung von Schwermetallspuren in Blutserum mittels Chelat-Ionenaustauscher