Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD and Edman degradation |
| |
Authors: | Sickmann A Blüggel M Kulke M Kremmin H Marx A Reske K Meyer H E |
| |
Affiliation: | Institut für Physiologische Chemie, Ruhr-Universit?t Bochum, Germany. |
| |
Abstract: | The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at -80 degrees C to prevent peptide losses. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|