Fabrication of a micro-optical lens using femtosecond laser 3D micromachining for two-photon imaging of bio-tissues |
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| Authors: | Lingling Qiao Fei He Yang Liao Koji Sugioka Change Pan |
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| Institution: | a State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, P.O. Box 800-211, Shanghai 201800, Chinab Graduate School of the Chinese Academy of Sciences, Beijing 100039, Chinac Laser Technology Laboratory, RIKEN—Advanced Science Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japand Institutes of Biomedical Sciences, Fudan University, P.O. Box 281-138, Shanghai 200032, China |
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| Abstract: | We report, for the first time to our knowledge, on the application of a micro-optical lens fabricated by three-dimensional (3D) femtosecond laser direct writing for two-photon fluorescence imaging of biological tissues. We show that the two-photon fluorescence images of a plant leaf tissue acquired with the micro-optical lens are comparable to that of a 5× objective lens. Our result represents an important step towards the application of micro-optical components fabricated by femtosecond laser micromachining in miniaturized nonlinear fluorescence microscopy applications, such as two-photon endoscopy. |
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| Keywords: | Femtosecond laser micromachining Micro-optical lens Surface roughness Two-photon imaging Bio-tissue |
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