Determination of Doxepin in human plasma using cloud‐point extraction with high‐performance liquid chromatography and ultraviolet detection |
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| Authors: | Mohammad Reza Baezzat Fatemeh Banavand Mahboobeh Tabandeh Ali Asghar Amiri Rogheye Pourghobadi |
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| Affiliation: | 1. Department of Chemistry, Payame Noor University, Tehran, I. R. of Iran;2. Faculty of Science, Department of Applied Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran |
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| Abstract: | A new straightforward method based on cloud‐point extraction has been developed, optimized, and validated for the determination of doxepin in human plasma by high‐performance liquid chromatography separation and UV detection. The nonionic surfactant Triton X‐114 was chosen as the extraction solvent. Chromatography separation was performed on a μBondapakR C18 column (4.6 mm id × 300 mm, 3 μm particle size), which was used for isocratic elution at a detection wavelength of 289 nm. Under the optimum conditions, the linear range of doxepin in human plasma was 0.1–0.9 μg/mL. Also, the detection limit, preconcentration factor, and enrichment factor were 0.08 μg/mL, 50, and 49.0, respectively. |
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| Keywords: | Cloud‐point extraction Doxepin High‐performance liquid chromatography Triton X‐114 |
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