Non‐polar lipids characterization of Quinoa (Chenopodium quinoa) seed by comprehensive two‐dimensional gas chromatography with flame ionization/mass spectrometry detection and non‐aqueous reversed‐phase liquid chromatography with atmospheric pressure chemical ionization mass spectrometry detection |
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Authors: | Chiara Fanali Marco Beccaria Simona Salivo Peter Tranchida Giusy Tripodo Sara Farnetti Laura Dugo Paola Dugo Luigi Mondello |
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Affiliation: | 1. University Campus Bio‐Medico, Rome, Italy;2. Dipartimento di Scienze del Farmaco e deiProdotti per la Salute, University of Messina, Messina, Italy;3. Diabetes Research Institute, Division of Cellular Transplantation of Surgery, University of Miami, Miami, FL, USA;4. Chromaleont S.r.l. A Spin‐off of the University of Messina, Messina, Italy |
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Abstract: | A chemical characterization of major lipid components, namely, triacylglycerols, fatty acids and the unsaponifiable fraction, in a Quinoa seed lipids sample is reported. To tackle such a task, non‐aqueous reversed‐phase high‐performance liquid chromatography with mass spectrometry detection was employed. The latter was interfaced with atmospheric pressure chemical ionization for the analysis of triacylglycerols. The main triacylglycerols (>10%) were represented by OLP, OOL and OLL (P = palmitoyl, O = oleoyl, L = linoleoyl); the latter was present in the oil sample at the highest percentage (18.1%). Furthermore, fatty acid methyl esters were evaluated by gas chromatography with flame ionization detection. 89% of the total fatty acids was represented by unsaturated fatty acid methyl esters with the greatest percentage represented by linoleic and oleic acids accounting for approximately 48 and 28%, respectively. An extensive characterization of the unsaponifiable fraction of Quinoa seed lipids was performed for the first time, by using comprehensive two‐dimensional gas chromatography with dual mass spectrometry/flame ionization detection. Overall, 66 compounds of the unsaponifiable fraction were tentatively identified, many constituents of which (particularly sterols) were confirmed by using gas chromatography with high‐resolution time‐of‐flight mass spectrometry. |
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Keywords: | Chenopodium Quinoa Lipids Mass spectrometry Triacylglycerols Unsaponifiable lipids |
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