Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography |
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Authors: | Changxia Liu Ruican Liu Peng Zhang Yiming Chen Tao Xu Fang Wang Tianwei Tan Chunqiao Liu |
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Affiliation: | Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China |
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Abstract: | The aim of present study is to develop an efficient and low‐cost method for capsaicin production isolated from capsaicinoids by macroporous resin adsorption chromatography. HZ816 resin has shown the best adsorption and desorption capacities for capsaicin among other resins. To optimize the operating parameters for separation, initial concentration, diameter‐to‐height ratio, mobile phase ratio, and crystallization method were investigated. When capsaicinoids solution (5 g/L) was loaded onto the column (diameter‐to‐height ratio = 1:12) with ethanol/1% w/w NaOH (4:6, v/v) as the mobile phase, capsaicin was purified most effectively. By using acid neutralization as the crystallization method, the purity of capsaicin improved from 90.3 to 99.5% with 82.3% yield. In conclusion, this study provides a simple and low‐cost method for the industrial‐scale production of high‐purity capsaicin. |
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Keywords: | Adsorption chromatography Capsaicin Capsaicinoids Chili Macroporous resin |
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