A fast isocratic liquid chromatography method for the quantification of xanthophylls and their stereoisomers |
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Authors: | Chamila Nimalaratne Daise Lopes‐Lutz Andreas Schieber Jianping Wu |
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Affiliation: | 1. Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, Edmonton, Alberta, Canada;2. Department of Nutritional and Food Sciences, University of Bonn, Bonn, Germany |
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Abstract: | A fast isocratic liquid chromatography method was developed for the simultaneous quantification of eight xanthophylls (13‐Z‐lutein, 13’‐Z‐lutein, 13‐Z‐zeaxanthin, all‐E‐lutein, all‐E‐zeaxanthin, all‐E‐canthaxanthin, all‐E‐β‐apo‐8’‐carotenoic acid ethyl ester and all‐E‐β‐apo‐8’‐carotenal) within 12 min, compared to 90 min by the conventional high‐performance liquid chromatography method. The separation was achieved on a YMC C30 reversed‐phase column (100 mm x 2.0 mm; 3 μm) operated at 20°C using a methanol/tert‐butyl methyl ether/water solvent system at a flow rate of 0.8 mL/min. The method was successfully applied to quantify lutein and zeaxanthin stereoisomers in egg yolk, raw and cooked spinach, and a dietary supplement. The method can be used for the rapid analysis of xanthophyll isomers in different food products and for quality control purposes. |
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Keywords: | Geometric isomers Isocratic elution Xanthophylls |
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