Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection |
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| Authors: | Jie Yang Yuanhong Wang Ran Zhang Tifngfu Jiang Zhihua Lv |
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| Institution: | 1. Key Laboratory of Marine Drugs, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China;2. Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, Ocean University of China, Qingdao, China |
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| Abstract: | Holothurian triterpene glycosides possess various kinds of biological activities, including antifungal, cytotoxic, hemolytic, cytostatic, and immunomodulatory effects. In this study, a rapid extraction method of triterpene glycosides from sea cucumbers using a small column of C18 solid phase was first developed. Furthermore, a novel high‐performance liquid chromatography method coupled with evaporative light scattering detection and electrospray ionization mass spectrometry was established for the determination of each triterpene glycosides from different sea cucumbers. Simultaneous separation of all kind of triterpene glycoside were achieved on a C18 column. A gradient of aqueous acetonitrile was applied, and the method was validated. The liquid chromatography method was applied to the online mass detection to identify the triterpene glycosides in the purified extraction of eight kinds of pulverized sea cucumber from the market of Qingdao, China. The negative mode of M–H]?/M–Na]? exclusively shown signals corresponding to the triterpene glycosides previously reported and the MS2 product ions of those ions indicate the specific structure of each triterpene glycoside. |
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| Keywords: | Electrospray ionization mass spectrometry Evaporative light scattering detection Sea cucumber Triterpene glycosides |
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