| 相干拉曼散射显微术 |
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| 引用本文： | 陈涛,虞之龙,张先念,谢晓亮,黄岩谊. 相干拉曼散射显微术[J]. 中国科学:化学, 2012, 0(1): 1-16 |
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| 作者姓名： | 陈涛 虞之龙 张先念 谢晓亮 黄岩谊 |
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| 作者单位： | [1]北京大学生物动态光学成像中心,北京100871 [2]北京大学工学院,北京100871 [3]Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA |
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| 基金项目： | 致谢 CARS和SRS显微实验得到了美国哈佛大学的Christian Freudiger博士、傅丹博士、Gary Holtom博士、BrianSaar博士、宋琳博士以及美国哥伦比亚大学的闵玮教授大力协助.部分实验样品的准备得到申洁的协助,部分实验数据取自2011年8月于北京大学举办的第八届相干拉曼散射显微学国际研讨会,何姣、纪旭和史晓做了大量工作.作者衷心地感谢来自以上人士的协助与支持.本文获得国家重点基础研究发展规划项目（2011CB809106）资助,特此一并致谢. |
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| 摘 要： | 相干拉曼散射显微术（Coherent Raman Scattering Microscopy）是一类植根于拉曼散射的光学显微成像方法,主要包含相干反斯托克斯拉曼散射（Coherent Anti-Stokes Raman Scattering,CARS）和受激拉曼散射（Stimulated Raman Scattering,SRS）两种方法.CARS/SRS显微术通过探测目标分子特定的振动来提供成像所需的衬度,通过非线性光学过程大大提高了检测的灵敏度,同时本征地具备三维成像能力.CARS和SRS显微术可以对脂类等不易被标记的物质成像,还可以很好地通过选择振动光谱,对生物体内特定小分子物质如药物等,以及生物大分子如核酸、蛋白质等进行无需标记的成像,因此成为极有潜力的活体（invivo）成像手段.本文主要介绍了CARS和SRS显微术的基本原理、实验操作及其在化学和生命科学中的应用.
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| 关 键 词： | 光学显微术 相干拉曼散射 反斯托克斯拉曼散射 受激拉曼散射 无标记成像 |
Coherent raman scattering microscopy |
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| CHEN Tao,YU ZhiLong,ZHANG XianNian,XIE XiaoLiang Sunney,HUANG YanYi. Coherent raman scattering microscopy[J]. Scientia Sinica Chimica, 2012, 0(1): 1-16 |
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| Authors: | CHEN Tao YU ZhiLong ZHANG XianNian XIE XiaoLiang Sunney HUANG YanYi |
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| Affiliation: | 1,2＊1 Biodynamic Optical Imaging Center,Peking University,Beijing 100871,China 2 College of Engineering,Peking University,Beijing 100871,China 3 Department of Chemistry and Chemical Biology,Harvard University,Cambridge,MA 02138,USA |
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| Abstract: | Coherent Raman Scattering Microscopy is an optical imaging method rooted in Raman Scattering.It involves Coherent Anti-Stokes Raman Scattering（CARS） and Stimulated Raman Scattering（SRS）.CARS and SRS offer chemical contrast via detecting vibration of chemical bonds.Both CARS and SRS are nonlinear optical processes,offering high sensitivity and 3-dimensional sectioning capability intrinsically.As label-free imaging technologies,CARS and SRS have been employed to observe substance that is hard to label,such as lipids and specific small molecules including metabolites,and large biomolecules including proteins and nucleic acids.These features also ensure CARS and SRS as promising imaging methods for in vivo studies.In this paper,we focus on the principle,methodology,as well as the applications of CARS and SRS microscopies in chemistry and life sciences. |
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| Keywords: | optical microscopy coherent raman scattering coherent anti-stokes raman scattering stimulated raman scattering label-free imaging |
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