Ion-exclusion chromatography of aliphatic car☐ylic acids on a cation-exchange resin by elution with polyvinyl alcohol |
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Authors: | Kazuhiko Tanaka Kazutoku Ohta James S. Fritz |
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Affiliation: | aNational Industrial Research Institute of Nagoya, 1-1 Hirate-cho, Kita-ku Nagoya 462, Japan bAmes Laboratory and Chemistry Department of Iowa State University, Ames, IA 50011-3020, USA |
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Abstract: | Ion-exclusion chromatography of aliphatic car☐ylic acids of different acidity (pKa) and hydrophobicity was investigated on a polystyrene-divinylbenzene (PS-DVB) based strongly acidic cation-exchange resin in the H+ form and conductivity detection by elution with polyvinyl alcohol (PVA). When water was used as an eluent, the resolution of the car☐ylic acids was very low and the peak accompanied a fronting depending on their hydrophobicities. Therefore, to improve the peak shape and the peak resolution, aqueous eluents containing PVAs (degrees of polymerization, n=500, 1500and2000) with many OH groups were tested for the ion-exclusion chromatographic separation of the car☐ylic acids. When aqueous eluents containing PVA were used, the fronting was decreased dramatically by the effect of increased hydrophilicity of the PS-DVB cation-exchange resin surface due to adsorption of OH group in PVA. The high resolution ion-exclusion chromatographic separation without the fronting and highly sensitive conductimetric detection of the car☐ylic acids was accomplished successfully by elution with a 0.2% PVA (n=1500)-10% methanol-water. |
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Keywords: | Mobile phase composition Car☐ylic acids Polyvinyl alcohol |
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