Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation |
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Authors: | Richardson Jonathan P Macmillan Derek |
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Affiliation: | Department of Chemistry, University College London, 20 Gordon Street, London, UK WC1H 0AJ. |
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Abstract: | Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, and ligation of the resulting fragments to afford biologically active proteins. |
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