| 氧化锆/聚亚甲基蓝修饰电极检测CaMV35S启动子基因 |
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| 引用本文： | 张娜,徐基贵,谈胜福,汪 丽,王 聪,杨柳青. 氧化锆/聚亚甲基蓝修饰电极检测CaMV35S启动子基因[J]. 中国无机分析化学, 2017, 7(2): 78-82. DOI: 10.3969/j.issn.2095-1035.2017.02.019 |
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| 作者姓名： | 张娜 徐基贵 谈胜福 汪 丽 王 聪 杨柳青 |
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| 作者单位： | 1. 宿州学院 化学化工学院,安徽 宿州,234000;2. 宿州学院 体育学院,安徽 宿州,234000 |
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| 基金项目： | 国家大学生创新创业项目(201510379037)；安徽省自然科学基金项目(1408085QB41)；宿州学院科研平台开放课题(2011YKF03，2015YKF16)；省级大学生创新创业训练项目(201510379051)；宿州学院大学生科研立项(KYLXLKYB16-04) |
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| 摘 要： | 制备了基于氧化锆(ZrO_2)/聚亚甲基蓝(PMB)修饰电极的无标记DNA传感器,用于转基因植物CaMV35S启动子基因的检测。探针DNA(ssDNA)通过ZrO_2和DNA的磷酸基的相互作用修饰到电极表面,以PMB氧化峰的示差脉冲伏安响应为检测信号,传感器和完全互补的DNA片段杂交后,PMB的氧化峰电流明显降低,当和完全不匹配的DNA片段杂交时,峰电流无明显变化。对于完全互补的DNA片段,在2.0×10~(-12)~2.0×10~(-8) mol/L浓度范围内峰电流的变化值和浓度的对数成良好的线性关系,检测限为4.1×10~(-13) mol/L(S/N=3)。所制备的传感器具有良好的稳定性、再生性和重现性,用于样品检测,结果令人满意。
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| 关 键 词： | 聚亚甲基蓝 氧化锆 DNA传感器 无标记 |
| 收稿时间： | 2016-10-08 |
| 修稿时间： | 2017-01-25 |
Preparation of Label-free Electrochemical CaMV35S Transgene Gene Sensor Based on ZrO2/poly(methelene blue) Modified Electrode |
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| ZHANG N,XU Jigui,TAN Shengfu,WANG Li,WANG Cong and YANG Liuqing. Preparation of Label-free Electrochemical CaMV35S Transgene Gene Sensor Based on ZrO2/poly(methelene blue) Modified Electrode[J]. Chinese Journal of Inorganic Analytical Chemistry, 2017, 7(2): 78-82. DOI: 10.3969/j.issn.2095-1035.2017.02.019 |
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| Authors: | ZHANG N XU Jigui TAN Shengfu WANG Li WANG Cong YANG Liuqing |
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| Affiliation: | Department of ChemistryChemical Engineering, Suzhou University, Suzhou,Anhui234000, China,Department of ChemistryChemical Engineering, Suzhou University, Suzhou,Anhui234000, China,Department of ChemistryChemical Engineering, Suzhou University, Suzhou,Anhui234000, China,Department of ChemistryChemical Engineering, Suzhou University, Suzhou,Anhui234000, China,Department of ChemistryChemical Engineering, Suzhou University, Suzhou,Anhui234000, China and Department of Physical Education, Suzhou University, Suzhou, Anhui 234000, China |
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| Abstract: | A ZrO2/poly(methelene blue) modified electrode was fabricated as a label-free DNA sensor used for CaMV35S transgene gene detection.Probe oligonucleotides (ssDNA) were immobilized on the ZrO2 thin films via the strong affinity between ZrO2 and phosphate groups. |
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| Keywords: | poly(methelene blue) ZrO2 DNA biosensor label-free |
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