| 高效液相色谱-电喷雾离子阱飞行时间质谱法鉴定乙酰甲喹及其代谢物 |
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| 引用本文： | 刘迎春,司红彬,贺利民,丁焕中,黄显会,陈建新,陈杖榴,曾振灵. 高效液相色谱-电喷雾离子阱飞行时间质谱法鉴定乙酰甲喹及其代谢物[J]. 分析化学, 2010, 38(1) |
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| 作者姓名： | 刘迎春 司红彬 贺利民 丁焕中 黄显会 陈建新 陈杖榴 曾振灵 |
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| 作者单位： | 华南农业大学兽医学院,广东省兽药研制与安全评价重点实验室,广州,510642 |
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| 基金项目： | 国家重点基础研究计划(973)项目(No.2009CB118805)资助 |
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| 摘 要： | 利用高效液相色谱分离、电喷雾-线性离子阱质谱法(LC-ESI/LTQ)、电喷雾-离子阱/飞行时间串联质谱法(LC-ESI/IT-TOF)鉴定鸡血浆中乙酰甲喹及其代谢物的结构。分别采用Hypersil Gold和Symmetry ShieldTM色谱柱,以含有0.01%甲酸水溶液(A)和甲醇(B)为流动相,梯度洗脱,电喷雾离子源(ESI)正离子模式进行检测。鸡口服给药乙酰甲喹(20mg/kg)后,翅下静脉采血,待分析血浆用乙腈-乙酸乙酯(3:2,V/V)混合液提取,吹干。残渣用30%甲醇水溶液复溶,上机检测。用Shimadzu′s Composition分子预测软件推测分子离子及其子离子质量,Xcalibur2.0.7软件分析线性离子阱质谱仪测得质谱图。鉴定出5种主要代谢物,分别为3-甲基-2-(1-羟基)乙基-喹啉-N1,N4-二氧化物(M1)、3-甲基-2-(1-羟基)乙基-喹啉-N4-一氧化物(M2)、3-甲基-2-乙酰基-喹啉-N4-一氧化物(M3)、3-甲基-2-乙酰基-喹啉(M4)和3-羟甲基-2-(1-羟基)乙基-喹啉-N1,N4-二氧化物(M5)。同时根据推测结果对代谢物M1～M4进行...
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| 关 键 词： | 乙酰甲喹 代谢物 高效液相色谱-电喷雾电离离子阱飞行时间串联质谱 结构鉴定 |
Identification of Mequindox and Its Metabolites by High Performance Liquid Chromatography Combined with Ion Trap-Time of Flight-Mass Spectrometry |
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| LIU Ying-Chun,SI Hong-Bin,HE Li-Min,DING Huan-Zhong,HUANG Xian-Hui,CHEN Jian-Xin,CHEN Zhang-Liu,ZENG Zhen-Ling. Identification of Mequindox and Its Metabolites by High Performance Liquid Chromatography Combined with Ion Trap-Time of Flight-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2010, 38(1) |
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| Authors: | LIU Ying-Chun SI Hong-Bin HE Li-Min DING Huan-Zhong HUANG Xian-Hui CHEN Jian-Xin CHEN Zhang-Liu ZENG Zhen-Ling |
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| Affiliation: | Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation;College of Veterinary Medicine;South China Agricultural University;Guangzhou 510642 |
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| Abstract: | The chemical structures of mequindox related metabolites in chicken plasma had been investigated using high performance liquid chromatography combined with linear ion trap quadrupole(LC-ESI/LTQ) and high performance liquid chromatography combined with ion trap-time of flight-mass spectrometry(LC-ESI/ITTOF).Samples were separated by Hypersil BDS C18 and symmetry ShieldTM columns,respectively,and 0.01% formic acid aqueous(A) and methanol(B) were used as mobile phase with gradient elution.Electrospray ionizati... |
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| Keywords: | Mequindox Metabolites High performance liquid chromatography Ion trap-time-of flight-mass spectrometry Structure identification |
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