| 正相液相色谱-蒸发光散射法测定食品中的石蜡 |
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| 引用本文： | 盛旋,胡艳云,张蕾,孙昊,郑屏,陶发荣,杨嫣嫣,杭吉.正相液相色谱-蒸发光散射法测定食品中的石蜡[J].分析化学,2009,37(12). |
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| 作者姓名： | 盛旋 胡艳云 张蕾 孙昊 郑屏 陶发荣 杨嫣嫣 杭吉 |
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| 作者单位： | 安徽出入境检验检疫局,合肥,230022 |
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| 摘 要： | 采用液相色谱(HPLC)结合蒸发光散射检测器(ELSD)对食品中的石蜡残留物进行了分析和检测.利用正相色谱柱对石蜡和非石蜡组分进行了分离,而无需对石蜡组分进行逐一分离.利用t检验,对构成石蜡的烷烃组分在ELSD检测器上的线性响应进行了显著性误差分析,结果显示,石蜡中烷烃组分具有相似的线性响应.以此为定量依据,实现了食品中石蜡含量的快速定量分析.并对HPLC-ELSD的检测和确证结果与GC-MS法进行了对比.方法的线性范围为10～500 mg/L,相关系数为0.9988;检出限为1 mg/L.以10, 50和100 mg/kg浓度水平添加石蜡时,其回收率在84.6%～105.4%之间,相对标准偏差为5.4%～7.2%.
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| 关 键 词： | 石蜡 蒸发光散射 液相色谱 食品 |
Application of Normal Phase Liquid Chromatography-Evaporative Light-Scattering Detection for Determination of Paraffin Wax in Food |
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| SHENG Xuan,HU Yan-Yun,ZHANG Lei,SUN Hao,ZHENG Ping,TAO Fa-Rong,YANG Yan-Yan,HANG Ji.Application of Normal Phase Liquid Chromatography-Evaporative Light-Scattering Detection for Determination of Paraffin Wax in Food[J].Chinese Journal of Analytical Chemistry,2009,37(12). |
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| Authors: | SHENG Xuan HU Yan-Yun ZHANG Lei SUN Hao ZHENG Ping TAO Fa-Rong YANG Yan-Yan HANG Ji |
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| Institution: | SHENG Xuan,HU Yan-Yun,ZHANG Lei,SUN Hao,ZHENG Ping,TAO Fa-Rong,YANG Yan-Yan,HANG Ji (Anhui Entry-Exit Inspection and Quarantine Bureau,Hefei 230022) |
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| Abstract: | A method was proposed for the separation and determination of paraffin waxes in food by HPLC-evaporative light scattering detection(ELSD).A normal-phase column was used to separate nonparaffinic and paraffinic materials without resolving the latter into individual components.The t-test method was adopted for the evaluation of mean difference between response factors of n-alkanes in paraffin waxes on ELSD detector.No mean difference was obtained between response factors,which can be used for quantitative det... |
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| Keywords: | Paraffin wax evaporative light-scattering liquid chromatography food |
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