Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway |
| |
Authors: | Kim Dong-Jo Chung Jun-Ho Ryu Eun-Kyeong Rhim Jung-Hyo Ryu Yoon-Sic Park So-Hyun Kim Kyung Tae Kang Heun-Soo Chung Hong-Keun Park Sang Chul |
| |
Institution: | Metabolic Engineering Laboratory Inc., Seoul National University College of Medicine, Seoul 110-799, Korea. |
| |
Abstract: | Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apoptosis based on the results of acridine orange/ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|