PSⅡ颗粒二聚体中类胡萝卜素向反应中心传能研究

运用瞬态荧光光谱技术在77 K低温下对PSⅡ颗粒复合物中类胡萝卜素（Car）分子的能量传递过程进行研究, 通过不同激发波长473 nm, 481 nm, 507 nm选择性激发PSⅡ颗粒复合物中色素分子, 得到PSⅡ颗粒二聚体中关于Car能量传递的三组时间组分：16.6 ps, 130~183 ps, 217~249 ps. 其中16.6 ps反映了LHCⅡ中的Car分子将能量通过中间体Cars、Chls分子传递到Chl639的过程; 130~183 ps为核心天线中的β-Car分子向RC的能量传递时间; 217~249 ps为LHCⅡ中Car481通过中间Chla分子向RC的传能时间.

Study on Energy Transfer from Carotenoid Moleculars to Reaction Centre in PhotosystemⅡdimer

To understanding the energy transfer from carotenoids molecular to reaction centre in PSII, PSII particle complex purified from spinach was investigated with ultrafast fluorescence spectroscopy in the case of selective excited carotenoid molecular with 473 nm, 481nm and 507 nm at 77 K. The data were analyzed by fluorescence decay time-fitting, and were compared with data of core complex got at the same condition. Three groups of time components about energy transfer of carotenoids were obtained: 16.6ps, 130~183 ps, 217~249 ps. The shortest lifetime component of 16.6 ps was the process of energy transfer from Car 473 molecular to Chl 639 in LHCII. The 130~183 ps were lifetime associated with the process of β-car molecular transfer the energy to RC by a series of intermediates and 217~249 ps reflected the process of Car_ 481 in LHCII transfer it′s energy to RC.