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Chemical Control of Yeast Cell Division by Cross‐Linked Shells of Catechol‐Grafted Polyelectrolyte Multilayers
Authors:Juno Lee  Sung Ho Yang  Seok‐Pyo Hong  Daewha Hong  Haeshin Lee  Hee‐Yoon Lee  Yang‐Gyun Kim  Insung S. Choi
Abstract:The chemical control of cell division has attracted much attention in the areas of single cell‐based biology and high‐throughput screening platforms. A mussel‐inspired cytocompatible encapsulation method for achieving a “cell‐division control” with cross‐linked layer‐by‐layer (LbL) shells is developed. Catechol‐grafted polyethyleneimine and hyaluronic acid are chosen as polyelectrolytes for the LbL process, and the cross‐linking of polyelectrolytes is performed at pH 8.5. Cell division is controlled by the number of the LbL nanolayers and cross‐linking reaction. We also suggest a new measuring unit, urn:x-wiley:10221336:marc201300444:equation:marc201300444-math-0001, for quantifying “cell‐division timing” based on microbial growth kinetics.
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Keywords:biomimetic synthesis  cell division  encapsulation  self‐assembly  yeast
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