线性扫描伏安法研究美托拉腙与DNA的相互作用 |
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引用本文: | 尚永辉,李华,孙家娟. 线性扫描伏安法研究美托拉腙与DNA的相互作用[J]. 分析测试学报, 2009, 28(11). DOI: 10.3969/j.issn.1004-4957.2009.11.025 |
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作者姓名: | 尚永辉 李华 孙家娟 |
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作者单位: | 咸阳师范学院,化学与化工学院,陕西,咸阳,712000;西北大学,分析科学研究所,陕西,西安,710069;西北大学,分析科学研究所,陕西,西安,710069;咸阳师范学院,化学与化工学院,陕西,咸阳,712000 |
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基金项目: | 国家自然科学基金资助项目,西北大学研究生交叉学科资助项目,咸阳师范学院专项科研基金资助项目 |
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摘 要: | 在NaOH(pH 10.0)介质中,美托拉腙分别在-1.810 V和-2.075 V处具有2个还原峰,DNA的加入导致美托拉腙的2个还原峰峰电位正移,峰电流下降,表明美托拉腙与DNA之间发生了相互作用,形成了非电活性的化合物.考察了时间、温度、扫描速率、离子强度等条件对该相互作用的影响;结合DNA对美托拉腙紫外吸收光谱的影响,推断美托拉腙分子与DNA分子的相互作用是通过嵌插方式结合.在优化条件下,反应体系在-2.075 V处的还原峰电流I_(pa)与DNA的质量浓度在6 ~36 mg/L范围内呈较好的线性关系,相关系数为0.994 8,检出限为3 mg/L;通过对反应机制的研究,得到美托拉腙与DNA间的结合常数β为1.334×10~4 L/mol,结合数为2.
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关 键 词: | 美托拉腙 脱氧核糖核酸(DNA) 电化学 相互作用 |
Linear Sweep Voltammetry Study on Interaction between Metolazone and DNA |
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Abstract: | Metolazone has two reduction peaks at - 1. 810 V and -2. 075 V in pH 10.0 NaOH solu-tion. After adding DNA, the peak current of metolazone decreased and the peak potential shifted positively, which indicated that metolazone could react with DNA and form an electrochemical inac-tive compound. Effects of reaction time, temperature, sweep rate and ionic intensity on the interac-tion between DNA and metolazone were studied, and ultraviolet-visible spectra of DNA - metolazone was investigated. The result indicated that the interaction between metolazone and DNA was mainly intercalative model. Under the optimal conditions, a good linear was observed between reduction peak current at - 2. 075 V and DNA concentration in the range of 6. 0 - 36 mg/L. The linear regres-sion equation was I_(pa)(10~(-5) A) = -0.041 97p +4. 322 5 with a correlation coefficient of 0.994 8, and the detection limit was 3.0 mg/L. The combination number and combination constant of DNA -metolazone were calcultaed to be 2 and 1. 334 × 10~4 L/mol, respectively. |
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Keywords: | metolazone DNA electrochemical interaction |
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