The dynamic kinetic resolution of 3-oxo-4-phenyl-β-lactam by recombinant E. coli overexpressing yeast reductase Ara1p |
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| Institution: | 1. University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, Sos. Panduri, no 90, 050663 Bucharest, Romania;2. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt;1. College of Agriculture, Henan University of Science and Techenology, Luoyang 471003, China;2. College of Life Science, Shaoxing University, Shaoxing 312000, China;3. College of Life Science, Anhui Agricultural University, Hefei 230036, China;4. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China |
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| Abstract: | Using a recombinant E. coli strain overexpressing yeast reductase Ara1p, we reduced racemic 3-oxo-4-phenyl-β-lactam to cis-(3S,4R)-3-hydroxy-4-phenyl-β-lactam as a single enantiopure product. The dynamic kinetic resolution occurred over the course of fermentation at pH 7. Under the same conditions, 3-oxo-4-(2-thiophenyl)-β-lactam 4 and 3-oxo-4-(2-furyl)-β-lactam 5 were not resolved. |
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