Trichosporon beigelli esterase (TBE): a versatile esterase for the resolution of economically important racemates |
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| Institution: | 1. Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/163-OC, A-1060 Vienna, Austria;2. Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, 117 Budapest, Hungary;3. Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164-SC, A-1060 Vienna, Austria;1. Institute of Chemistry, São Paulo State University, UNESP, C.P. 355, 14801-970, Araraquara, SP, Brazil;2. Centro de Pesquisas René Rachou/FIOCRUZ, Av. Augusto de Lima 1715, 30190-002, Belo Horizonte, MG, Brazil;1. Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di Sotto 10, 06123 Perugia, Italy;2. Dipartimento di Farmacia e Biotecnologie, Università di Bologna, Via Belmeloro 6, 40126 Bologna, Italy;3. Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy;4. Dipartimento di Farmacia, Università di Napoli “Federico II”, Via D. Montesano 49, 80131 Napoli, Italy;5. Department of Chemistry, University of Southern California, Los Angeles, CA 90089-0744, USA;6. Dipartimento di Chimica “G. Ciamician”, Alma Mater Studiorum-Università di Bologna, Via Selmi 2, 40126 Bologna, Italy;7. Dipartimento di Scienze, Università della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy |
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| Abstract: | A hydrolase producing strain Trichosporon beigelli esterase (TBE) isolated from local cottage cheese in its native form has displayed versatility and high efficacy in the kinetic resolution of a wide range of economically important substrates, which include racemic secondary alcohols, such as 1-(6-methoxy-2-naphthyl)ethanol (E ∼ 316), 1-(3,4-methylenedioxyphenyl)ethanol and pentanol (E ∼ 180 and 156 resp.), and alkyl esters of carboxylic acids such as ibuprofen (E ∼ 340), 2-(benzylthio)propanoic acid (E ∼ 1000). In other substrates such as in the primary alcohol 2-(6-methoxy-2-naphthyl)propan-1-ol and carboxylic acids such as 2-(5-bromo-6-methoxy-2-naphthyl)propanoic acid, 2-(2-naphthyloxy)propanoic acid, and substituted 2-thiopropanoic acids, it displayed moderate to low selectivity. Commercial lipases such as CCL, PPL, and PSL were also used in the resolution of the substrates for comparative studies. |
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