氢键吸附树脂的合成及高纯度沙棘叶黄酮的制备

针对黄酮分子的酚羟基结构特点,有目的地选择了4种结构不同的商品化树脂,考察了它们对沙棘叶中黄酮类有效成分的吸附能力和吸附选择性.在此基础上优化了树脂结构设计方案,合成了具有氨基功能基、可形成氢键作用、疏水性可调变的大孔吸附树脂.以沙棘叶粗提物为原料,考察了树脂骨架疏水性、功能基间隔臂长短等对树脂纯化效果的影响规律.研究结果表明,当吸附发生在水体系中,一定强度的疏水性作用是树脂与吸附质之间形成氢键的必要条件,对于尺寸较大的黄酮分子,氢键功能基的间隔臂长短也显著影响了树脂对它的吸附能力.最后,我们选择了疏水性和氢键功能基间隔臂长短适宜的XM20-2树脂,对吸附和洗脱条件进行了优化,将其用于沙棘叶粗提物中黄酮类有效成分的进一步纯化,可将黄酮纯度从粗提物中的10.4%提高到50%以上,且树脂具有很好的重复使用性.

SYNTHESIS OF HYDROGEN-BONDING POLYMERIC ADSORBENTS AND PREPARATION OF HIGHLY PURIFIED FLAVONOIDES EXTRACTS FROM SEA BUCKTHORN LEAVES

Based on results of adsorption property of four selected commercial adsorbents,a series of high selectivity polymeric adsorbents were synthesized based on the copolymerization of methyl acrylate (MMA) and divinylbenzene (DVB).The amino functional group was introduced into the adsorbent matrix to produce the hydrogen-bonding interaction with the flavonoides,which has the typical phenolic hydroxyl structure.The influences of the hydrophobic intensity of the adsorbent and the spacer arm length of the functional group on the adsorption property were investigated.When the adsorption was carried out in the aqueous environment reasonable hydrophobic affinity is necessary to assist the hydrogen bonding.In addition,the length of the spacer arm of the functional group affected the adsorption ability owing to the large size of flavonoides molecule.The adsorbent XM20.2 showed the optimal adsorption capacity and selectivity to the flavonoides.Experimental parameters for adsorption and elution were optimized.After purification of the crude extract of sea buckthorn leaves,the content of flavonoides increased from 10.4% to above 50%.The adsorbent showed good reproducibility.