| 地鳖纤溶活性蛋白(EFP)的分离纯化、红外光谱分析及抑制鸡胚尿囊膜(CAM)血管生成研究 |
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| 引用本文： | 李穗晶,韩雅莉,张冬梅,刘浩,黎子蔚.地鳖纤溶活性蛋白(EFP)的分离纯化、红外光谱分析及抑制鸡胚尿囊膜(CAM)血管生成研究[J].高等学校化学学报,2009,30(10):1998-2002. |
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| 作者姓名： | 李穗晶 韩雅莉 张冬梅 刘浩 黎子蔚 |
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| 作者单位： | 广东工业大学轻工化工学院生物工程系, 广州 510006 |
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| 摘 要： | 以传统中药材地鳖为原料, 采用匀浆、盐析、硫酸铵分段沉淀、透析和DEAE-52纤维素离子层析等纯化方法, 从其体内分离纯化得到一种纤溶活性蛋白(Fibrinolyric protein of Eupolyphaga sinensis Walker, EFP), 采用SDS-PAGE电泳法对该蛋白进行了分子量和纯度测定, 结果表明, 从地鳖中提取纯化的EFP为相对分子量为41300的单一成分, 具有明显的纤溶活性, 由蛋白质和糖的红外光谱特征吸收峰可推断EFP为一种糖蛋白. EFP对鸡胚尿囊膜新生血管生成有良好的抑制作用, 比阳性对照组(地塞米松组)对鸡胚生长发育影响要小. 地鳖虫纤溶活性蛋白组分具有抑制血管生成的作用, 有可能用于肿瘤治疗.
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| 关 键 词： | 地鳖纤溶活性蛋白 血管生成 红外光谱 |
| 收稿时间： | 2009-01-13 |
Studies on the Purification,Infrared Spectroscopic Analysis and Antiangiogenesis Effects of Fibrinolyric Protein(EFP) from Eupolyphage sinesis Walker |
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| LI Sui-Jing,HAN Ya-Li,ZHANG Dong-Mei,LIU Hao,LI Zi-Wei.Studies on the Purification,Infrared Spectroscopic Analysis and Antiangiogenesis Effects of Fibrinolyric Protein(EFP) from Eupolyphage sinesis Walker[J].Chemical Research In Chinese Universities,2009,30(10):1998-2002. |
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| Authors: | LI Sui-Jing HAN Ya-Li ZHANG Dong-Mei LIU Hao LI Zi-Wei |
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| Institution: | Bioengineering Department, Faculty of Light and Chemical, Guangdong University of Technology, Guangzhou 510006, China |
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| Abstract: | A novel of fibrinolytic protein(EFP) was separated and purified by homogenate,salting-out,ammonium sulfate fractionation,DEAE-52 cellulose column chromatography from fresh tradional chinese medicine Eupolyphaga sinensis Walker.The molecular weight and purity of EFP were detected by sulfate-polyacry-lamide gel electrophoresis analysis.Thin polyacrylamide fibrin plate assay was used to test the fibrinolysin activity of EFP.The key functional group of EFP was analyzed by infrared spectroscopy.Chicken embryo ch... |
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| Keywords: | Fibrinolyric protein of Eupolyphaga sinensis Walker(EFP) Angiogenesis Infrared spectroscopy |
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