| Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode |
| |
| 引用本文： | 王树青,陈峻,林祥钦. Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode[J]. 中国化学, 2004, 22(4): 360-364. DOI: 10.1002/cjoc.20040220409 |
| |
| 作者姓名： | 王树青 陈峻 林祥钦 |
| |
| 作者单位： | Department of Chemistry，University of Science and Technology of China，Department of Chemistry，University of Science and Technology of China，Department of Chemistry，University of Science and Technology of China Hefei，Anhui 230026，China，Hefei，Anhui 230026，China，Hefei，Anhui 230026，China |
| |
| 基金项目： | theNationalNaturalScienceFoundationofChina(No.20075025). |
| |
| 摘 要： | IntroductionAmperometricbiosensorofhydrogenperoxideisofpracticalimportancebecauseofitswideapplicationsinchemical,biological,clinical,environmentalandmanyotherfields.Forimprovementofsensor抯quality,vari-ouskindsofchemicalmodificationmethodshavebeendevelopedforreducingredoxoverpotentialsofH2O2atelectrodesurfaces,increasingthedetectionsensitivity,linearrange,stabilityandlivetime.Ithasbeenshownthattheuseofsub-micrometersizedmetalparticlessuchasPt-blackcansignificantlyimprovethequalityofthebiosens…
|
| 关 键 词： | 过氧化氢 安培计 生物传感器 玻璃态碳电极 亚甲基蓝 山葵过氧化物酶 |
Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode |
| |
| WANG,Shu-Qing CHEN,Jun LIN,Xiang-Qin. Amperometric Biosensor for Hydrogen Peroxide Based on Electrodeposited Sub-micrometer Gold Modified Glassy Carbon Electrode[J]. Chinese Journal of Chemistry, 2004, 22(4): 360-364. DOI: 10.1002/cjoc.20040220409 |
| |
| Authors: | WANG Shu-Qing CHEN Jun LIN Xiang-Qin |
| |
| Affiliation: | WANG,Shu-Qing CHEN,Jun LIN,Xiang-Qin*Department of Chemistry,University of Science and Technology of China,Hefei,Anhui 230026,China |
| |
| Abstract: | A new type of hydrogen peroxide amperometric biosensor was fabricated based on electrochemically deposited sub‐micrometer Au particles (sm‐Au) on a glassy carbon electrode (GCE). Electrochemical deposition condition was optimized for obtaining uniformly distributed sub‐micrometer sized Au array on the electrode surface. The hydrogen peroxide sensor was fabricated by adsorbing phenothiazine methylene blue (MB) molecules on the surface of sm‐Au and covering a cross‐linked horseradish peroxidase (HRP) layer, labeled as HRP/MB/sm‐Au/GCE. The characteristics of this biosensor were evaluated with respect to applied potential and pH. The amperometric response of the sensor was linear to the H2O2 concentration over a wide range of 9.9×10?6—1.11×10?2 mol/L. A detection limit (s/n=3) of 3.0×10?6 mol/L H2O2 was estimated for a sampled chronoamperometric detection at 1.5 min after potential step of 200 to ?400 mV vs. SCE. The immobilized MB molecules shuttled electrons at α= 0.77 and an apparent electron transfer rate constant of k05 =0.053 s?1. Interference of ascorbic acid, dopamine and uric acid was investigated. This sensor has very good stability and reproducibility for long‐term use. |
| |
| Keywords: | hydrogen peroxide amperometric biosensor sub-micrometer gold glassy carbon electrode me-thylene blue horseradish peroxidase |
| 本文献已被 CNKI 等数据库收录！ |
