Fluorescent sensing platform for low-cost detection of Cu2+ by coumarin derivative: DFT calculation and practical application in herbal and black tea samples |
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Authors: | Tahir SAVRAN Abdurrahman KARAG
Z ükriye Nihan KARUK ELMAS Duygu AYDIN Furkan
ZEN Kenan KORAN Fatma Nur ARSLAN Ahmet Orhan G
RGÜLÜ brahim YILMAZ |
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Institution: | 1. Department of Chemistry, Kamil Özdağ Science Faculty, Karamanoğlu Mehmetbey University, Karaman, Turkey ; 2. Department of Mathematics and Science, Faculty of Education, Akdeniz University, Antalya, Turkey ; 3. Department of Chemistry, Faculty of Science, Fırat University, Elazığ, Turkey |
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Abstract: | A fluorogenic probe based on a coumarin-derivative for Cu2+ sensing in CH3CN/H2O media (v/v, 95/5, 5.0 μM) was developed and applied in real samples. 3-(4-chlorophenyl)-6,7-dihydroxy-coumarin (MCPC) probe was obtained by synthetic methodologies and identified by spectral techniques. The probe MCPC showed remarkable changes with a “turn-off” fluorogenic sensing approach for the monitoring of Cu2+ at 456 nm under an excitation wavelength of 366 nm. The response time of the probe MCPC was founded as only 1 min. The detection limit of the probe MCPC was recorded to be 1.47 nM. The binding constant and possible stoichiometric ratio (1:1) values were determined by Benesi-Hildebrand and Job’s plot systems, respectively. The mechanism of the probe MCPC with Cu2+ was further confirmed by ESI-MS and FT-IR analyses, as well as supported by theoretical calculations. Furthermore, the probe MCPC was successfully employed for the practical applications to sense Cu2+ in different herbal and black tea samples. The proposed sensing method was also verified by ICP-OES method. |
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Keywords: | Coumarin fluorescent sensor copper DFT tea samples |
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