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Development of an HPLC method for the identification and dosage of non-allowed substances in cosmetic products. Part I: local anaesthetics and antihistaminics |
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| Authors: | Rita Porrà Simona Berri Luigi Gagliardi Paola Chimenti Arianna Granese Daniela De Orsi Irene Carpani Domenica Tonelli |
| Institution: | (1) Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanità, Rome, Italy;(2) Dipartimento di studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Università la Sapienza, Rome, Italy;(3) Dipartimento di Chimica Fisica ed Inorganica, Università di Bologna, Viale del Risorgimento 4, 40136 Bologna, Italy |
| Abstract: | An HPLC method with ultraviolet detection coupled with a solid-phase extraction sample clean up was developed for the analysis of five local anaesthetics and four antihistaminics in cosmetic products. The presence of these compounds in commercial cosmetic samples is fordbidden. Extracts from real samples were applied to a solid-phase extraction C18 cartridge, and the analytes were eluted with 8:2 (v/v) acetonitrile/water containing 1 trifluoroacetic acid. HPLC separation was then performed for the identification and determination of the analytes using a Purospher RP-18 column, two gradient eluting systems and a photodiode-array detector. The accuracy of the method was verified by spiking experiments on home-made cosmetic samples. The analytical recoveries were satisfactory. |
| Keywords: | HPLC Local anaesthetics Antihistaminics Cosmetic products |
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