牛血清白蛋白与甲基橙结合反应的研究

用分光光度法研究了牛血清白蛋白与甲基登在酸性溶液中的结合反应，认为两者通过非共价键结合，研究了溶液吸光 度与组分浓度的关系，对Ｓｃａｔｃｈａｒｄ模型用于自理蛋白质染色反应时存在的总是作了讨论，提出了表观结合常数的概念，推导了求算表观结合常数和最大结合数的线性回归公式。

Interaction of Bovine Serum Albumin with Methyl Orange in Acidic Solution

The interaction of methyl orange(MO)with bovine serum albumin(BSA)in acidic solution was investigated by a spectrophotometric method. It was considered that the combination of BSA with MO is due to the noncovalent binding forces.The color change during the binding process is because of the transformation of MO from free-acidic form into bound-basic form.The relationship between absorbance and concentrations of MO species was studied.The Scatchard model was discussed in a new point of view.Two conditional constants,apparent binding constant and maximum binding number,were proposed to express the binding ability of MO on BSA,and two linear regression equations were derived to estimate these two parameters.It was found that sodium chloride concentration and acidity have significant effect on the binding reaction.