ATR-IR spectroscopy as applied to nucleic acid films |
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Authors: | Stepanyugin Andriy V Samijlenko Svitlana P Martynenko Olena I Hovorun Dmytro M |
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Institution: | Institute of Molecular Biology and Genetics, Ukrainian National Academy of Sciences, 150 vul. Zabolotnoho, 03143 Kyiv, Ukraine. |
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Abstract: | For the first time the ATR technique was applied to obtain IR absorption spectra of DNA and RNA dry films. There was worked out procedure of the nucleic acid removal from germanium plate, which obviously was a main obstacle to application of ATR-IR spectroscopy to nucleic acids. This technique of IR spectroscopy was applied to confirmation of RNA tropism of aurin tricarboxylic acid observed by molecular biological methods. |
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