| Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;中国科学院"百人计划" |
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| 摘 要： | Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate and N,N′-memylenebisacrylamid in the presence of trinary porogens, including 1,4-butanediol, dodecanol and dimethyl sulphoxide. With Ampholine immobilized on the monolith by chemical bonding according to their pIs, the monolithic immobilized pH gradient (M-IPG) was prepared, and applied to the separation of four standard proteins. Compared with polyacrylate based M-IPG, the hydrophilicity of the new material was improved. It could not only avoid the adsorption of proteins, but also make the synthesized procedure simple, which showed great potential in the analysis of proteins.
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| 收稿时间： | 7 December 2005 |
| 修稿时间： | 14 September 2006 |
Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis |
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| Authors: | Zhu GuiJie Zhang WeiBing Zhang LiHua Liang Zhen Zhang YuKui |
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| Institution: | 1. National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;Graduate University of Chinese Academy of Sciences, Beijing 100039, China
2. National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
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| Abstract: | Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate and N,N′-memylenebisacrylamid in the presence of trinary porogens, including
1,4-butanediol, dodecanol and dimethyl sulphoxide. With Ampholine immobilized on the monolith by chemical bonding according
to their p/s, the monolithic immobilized pH gradient (M-IPG) was prepared, and applied to the separation of four standard proteins. Compared
with polyacrylate based M-IPG, the hydrophilicity of the new material was improved. It could not only avoid the adsorption
of proteins, but also make the synthesized procedure simple, which showed great potential in the analysis of proteins.
Supported by the State Key Fundamental Research Program (Grant No. 2001CB51020), National Natural Science Foundation of China
(Grant Nos. 20405104 and 20435020) and 100 Talents Project of Chinese Academy of Sciences |
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| Keywords: | capillary isoelectric focusing monolith immobilized pH gradient proteins |
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