O-(硫杂蒽酮-[2]-基)-氧乙酸的合成、表征及与DNA作用的研究

合成了O-(硫杂蒽酮-[2]-基)-氧乙酸，并用元素分析、IR、1H NMR和13C NMR等方法对其结构进行了表征。用光谱学方法研究了该化合物与ct-DNA的相互作用。结果表明，在pH 7.38的模拟体液条件下，该化合物的紫外-可见吸收光谱随ct-DNA浓度的增加，表现出减色效应，使ct-DNA的圆二色谱正负峰的吸收强度有所降低。该化合物的荧光强度被ct-DNA显著猝灭，猝灭常数为1.25×104 L·mol-1,当ct-DNA的浓度在0～12.0 mg·L-1范围内变化时，荧光猝灭值与ct-DNA的浓度呈现良好的线性关系。该化合物对ct-DNA的检出限为1.63 mg·L-1。据此，O-(硫杂蒽酮-[2]-基)-氧乙酸是一种可用于测定ct-DNA浓度的新试剂，与ct-DNA的作用有嵌入和静电吸引两种方式。

Synthesis and Characterization of Thiaxanthon-2-yl-oxyacetate and Its Combination with ct-DNA

The thiaxanthon-2-yl-oxyacetate was synthesized,and its structure was characterized by elemental analysis,IR,1H NMR,and 13C NMR spectra. It was found from the experiment that thiaxanthon-2-yl-oxyacetate had very intense fluorescence,so spectroscopy methods were used to study the interactions of thiaxanthon-2-yl-oxyacetate and ct-DNA. The ultraviolet absorption intensity of thiaxanthon-2-yl-oxyacetate decreased as the concentration of ct-DNA increased at pH 7.38. There was some debased intensity both in the negative and positive spectra of CD spectra of ct-DNA when thiaxanthon-2-yl-oxyacetate interacted with ct-DNA,and quenching effect occurred in the fluorescence spectrum of thiaxanthon-2-yl-oxyacetate. The quenching constant is 1.25×104 L·mol-1,when the concentration of ct-DNA changed from 0 to 12.0 mg·L-1,and the connection between the quenching data and the concentration of ct-DNA shows a favorable linear relationship. The detection limit was 1.63 mg·L-1,so the compound is a new reagent for the measurement of ct-DNA. The interaction of TYO and ct-DNA was insetted and static.