Characterization of a Bio-sourced,Fluorescent, Ratiometric pH Indicator with Alkaline pKa |
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| Authors: | Samar Alheety Domenic Valenti Nirvani Mujumdar Nakita Ellis Andres D. Campiglia James K. Harper Emily C. Heider |
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| Affiliation: | 1. University of Central Florida, Orlando, FL;2. Utah Valley University, Orem, UT;3. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT |
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| Abstract: | Utilizing organisms as sources of fluorophores relieves the demand for petroleum feedstock in organic synthesis of fluorescent products, and endophytic fungi provide a promising vein for natural fluorescent products. We report the characterization of a pH-responsive fluorophore from an endophytic fungus isolated from sand pine. The endogenous fluorescence of the live organism was measured using fluorescence microscopy. Computational interpretation of the spectra was accomplished with time-dependent density functional theory methods. The combined use of experimental and theoretically predicted spectra revealed the pH equilibria and photoexcited tautomerization of the natural product, 5-methylmellein. This product shows promise both as a stand-alone pH-indicating fluorophore, with alkaline pKa, and as "green" feedstock for synthesis of custom fluorophores. |
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