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Fluorimetric determination of tetracycline and anhydrotetracycline
Authors:Wen-Bao Chang   Yi-Bing Zhao   Yun-Xiang Ci  Lin-Yun Hu
Affiliation:(1) Department of Chemistry, Peking University, 100871 Beijing, PR China;(2) Department of Chemistry, Capital Institute of Medicine, 100054 Beijing, PR China
Abstract:Summary A rapid fluorimetric method employing the complexes of Al3+ with tetracycline (TC) and anhydrotetracycline (ATC) has been used for the measurement of the concentrations of TC and ATC in about 10–7–10–6 mol/l. The assay offers simplicity and rapidly compared with other methods. The recoveries in the determination of synthetic samples are in the range of 95–103% for TC and 101–120% for ATC. Several determinations of TC and ATC in serum and urine have been carried out with satisfactory results.
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