近红外光谱技术在检测转基因油菜籽中芥酸和硫甙上的应用研究

随着转基因食品的迅速发展，人们越来越关心其食用安全性。以转基因油菜及其亲本为实验材料，借助于近红外光谱仪对转基因油菜及其亲本中的芥酸和硫甙进行了测定分析：扫描区间为12 000～4 000 cm-1,分辨率为4 cm-1,扫描次数为64次。结果显示近红外检测样品的结果与通用方法结果相近，说明通过扫描光谱加上数学和计算机软件分析，非常准确、方便的测定了转基因油菜中的芥酸和硫甙。结果表明，转基因油菜中的芥酸含量明显高于亲本中的含量，芥酸含量是非转基因亲本1.5～2.0倍;硫甙含量也表现出芥酸相同的规律，转基因油菜中显著高于其相应亲本，是亲本含量的1.3～1.51倍以上。所以，在转基因作物的育种过程中，在改善个别性状的同时，如何保持其他优良性状应当引起重视。

Detection of Erucic Acid and Glucosinolate in Intact Rapeseed by Near-Infrared Diffuse Reflectance Spectroscopy

With the rapid development of transgenic food,more and more transgenic food has been pouring into the market,raising great concern about transgenic food's edible safety.To analyze the content of erucic acid and glucosinolate in transgenic rapeseed and its parents,all the seeds were scanned intact by continuous wave of near infrared diffuse reflectance spectrometry ranging from 12 000 to 4 000 cm~(-1) with a resolution of 4 cm~(-1) and 64 times of scanning.Bruker OPUS software package was applied for quantification,while the results were compared with the standard methods.The results showed that the method of NIRS was very precise,which proved that infrared diffuse reflectance spectroscopy can be applied to detect the toxins in transgenic food.On the other hand,the results also showed that the content of erucic acid in transgenic rapeseeds is 0.5-1.0 times higher than that of their parents,and the content of glucosinolate in transgenic rapeseeds is 1.3-0.6 times higher than that of their parents.