Large-scale separation of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by pH-zone-refining countercurrent chromatography |
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| Authors: | Wang Xiao Geng Yanling Li Fuwei Liu Jianhua |
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| Institution: | Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong, China. wangx@keylab.net |
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| Abstract: | pH-Zone-refining countercurrent chromatography was successfully applied to the separation of salvianolic acid B from the Chinese medicinal plant, Salvia miltiorrhiza Bunge, using a multilayer coil planet centrifuge. A 2.0 g quantity of sample was separated using the following two-phase solvent system: methyl tert-butyl ether (MtBE)-water, 10 mM TFA in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by HPLC and ESI-MS. The separation yielded 572 mg of the main component of salvianolic acid B with a purity of 94.1%. |
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| Keywords: | pH‐Zone‐refining countercurrent chromatography Preparative chromatography Salvia miltiorrhiza Bunge Salvianolic acid B |
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