Structural analysis of three-spin systems of photosystem II by PELDOR |
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| Authors: | A Kawamori N Katsuta H Hara |
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| Institution: | 1. School of Science and Technology, Kwansei Gakuin University, 665-1337, Sanda, Japan
2. Bruker Biospin K.K., Tsukuba, Japan
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| Abstract: | The pulsed electron electron double resonance (PELDOR) pulse sequence is applied to a three-spin system consisting of three radicals (Y D · , Y Z · and Q A ? ) generated in spinach PS II. The distance between YZ and QA has been determined to be 3.4 nm with the previously derived distances of the other radical pairs, 2.9 nm for Y D · -Y Z · and 3.9 nm for Y D · -Q A ? . This distance has been derived from the Y Z · -Q A ? radical pair trapped in YD-less mutants ofChlamydomonas reinhardtii. Furthermore the method was applied to the Y D · -Q A ? -Chl Z + system to find the unknown distance between QA and ChlZ. The derived distance was 3.4 nm. A triangular configuration was found in the membrane system that gives the relative positions of the electron transfer components. |
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