| 新型壳聚糖液相色谱填料及其在生物分子分离中的应用 |
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| 引用本文： | 王俊德,王宇锦,孙朝晖.新型壳聚糖液相色谱填料及其在生物分子分离中的应用[J].色谱,1994,12(5):327-329. |
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| 作者姓名： | 王俊德 王宇锦 孙朝晖 |
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| 作者单位： | 中国科学院大连化学物理研究所 |
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| 基金项目： | 国家自然科学基金的资助 |
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| 摘 要： | 研制了壳聚糖键合硅胶和壳聚糖涂层交联硅胶填料。考察了这两种液相色谱填料对几种小肽、蛋白质和单糖的色谱保留行为。发现它们对小肽有一定的分离能力，在蛋白质的分离中有一定特色，但对单糖的保留不大。
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| 关 键 词： | 单糖. 蛋白质 高效液相色谱 壳聚糖固定相 小肽 |
Preparation of Chitosan-Bonded and Chitosan-Coated-High Performance Liquid Chromatography(HPLC)Packings and Their Applications to Separation of Biopolymers |
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| Wang Junde,Wang Yujin and Sun Zhaohui.Preparation of Chitosan-Bonded and Chitosan-Coated-High Performance Liquid Chromatography(HPLC)Packings and Their Applications to Separation of Biopolymers[J].Chinese Journal of Chromatography,1994,12(5):327-329. |
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| Authors: | Wang Junde Wang Yujin and Sun Zhaohui |
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| Abstract: | he preparation of chitosan-bonded and chitosan-coated HPLC packings and their applications to the sepa-ration of small peptides,proteins and sugars were reported in the paper.Under isocratic elution,the effectsof mobile phase parameters,such as,pH value and concentration of salts(NH _4)_2SO_4] on the elution of pro-teins were examined.The results showed that the chitosan-bonded pack ing gives a good resolution for thepeptides and both chitosan-silica packings exhibit a certain feature for protein retentions,but they have lessseparation ability for sugars. |
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| Keywords: | high performance liquid chromatography chitosan stationary phase small peptide protein monosaccharide |
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