柱前衍生毛细管电泳-电化学检测组胺

本文采用邻苯二甲醛（OPA）为柱前衍生化试剂，用毛细管电泳-电化学检测（CE—ED）法测定酱油中组胺的含量。以直径300μm的碳圆盘电极为工作电极，50mmol／L硼砂（pH8．7）为运行缓冲液，对组胺和组氨酸的分离检测条件进行优化。在优化条件下，组胺和组氨酸二组分可在12min内完全分离，检出限分别为7．5×10^-7g／mL和5．0×10^-4g／mL。该方法已经成功用于实际样品的检测。

Determination of Histamine by Pre-column Derivatization Capillary Electrophoresis with Electrochemical Detection

Using OPA as the pre-column derivatizing reagent, histamine in soy was determined by capillary electrophoresis with electrochemical detection (CD-ED) in this paper. For studying the determination condition of histamine and histidine, a 300 μm diameter carbon disk electrode was used as the working electrode and 50 mmoL/L borax solution (pH=8.7) as running buffer. Under the optimum conditions, two analytes were separatered within 12 min with detection limits of 7.5×10-7 g/mL and 5.0×10-6 g/mL, respectively. This method was successfully used for the analysis of histamine in real samples.