Immobilization and Characterization of Lactate Dehydrogenase on TEOS Derived Sol-Gel Films |
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Authors: | Kumaran Ramanathan M.N. Kamalasanan B.D. Malhotra D.R. Pradhan S. Chandra |
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Affiliation: | (1) Display Devices Group, National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi-, 110 012, India |
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Abstract: | Physical adsorption and physical entrapment techniques have been utilized for the immobilization of lactate dehydrogenase (LDH) on tetraethylorthosilicate (TEOS) derived sol-gel films. The enzyme (LDH) activity has been assayed as a function of time, temperature, pH and pyruvate concentration. The results of photometric measurements used for monitoring the reaction yield a response time of about 1 min, linearity over a concentration range of 0–1.5 × 10-3 M and detection limit of 5 × 10-5 M. The TEOS sol-gel films containing LDH have been found to be stable for about 30 days at temperatures 4 to 10°C. |
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Keywords: | lactate dehydrogenase sol-gel biosensor lactic acid |
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