<Emphasis Type="Italic">Ex vivo</Emphasis> infection of human embryonic spinal cord neurons prior to transplantation into adult mouse cord |
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| Authors: | Gábor?Márton Dóra?Tombácz Judit?S?Tóth András?Szabó Zsolt?Boldogk?i ádám?Dénes ákos?Hornyák Antal?Nógrádi |
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| Institution: | (1) Department of Ophthalmology, Faculty of General Medicine, University of Szeged, Szeged, Hungary;(2) Department of Medical Biology, Faculty of General Medicine, University of Szeged, Szeged, Hungary;(3) Laboratory of Molecular Neuroendocrinology, Insitute of Experimental Medicine, Budapest, Hungary;(4) Department of Virology, Central Veterinary Institute, Budapest, Hungary;(5) Dept. of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK |
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| Abstract: | Background Genetically modified pseudorabies virus (Prv) proved suitable for the delivery of foreign genes to rodent embryonic neurons
ex vivo and maintaining foreign gene expression after transplantation into spinal cord in our earlier study. The question arose of
whether human embryonic neurons, which are known to be more resistant to Prv, could also be infected with a mutant Prv. Specifically,
we investigated whether a mutant Prv with deleted ribonucleotide reductase and early protein 0 genes has the potential to
deliver marker genes (gfp and β-gal) into human embryonic spinal cord neurons and whether the infected neurons maintain expression
after transplantation into adult mouse cord. |
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