Determination of molecular weight of silk fibroin by non-gel sieving capillary electrophoresis |
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Authors: | Wei Wei Zhang Yaopeng Shao Huili Hu Xuechao |
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Affiliation: | Donghua University, College of Material Science and Engineering, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai 201620, People's Republic of China. |
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Abstract: | A simple non-gel sieving capillary electrophoresis (NGSCE) method was established to determine the MW of silk fibroin using CE. The background electrolyte with a pH of 8.8 was based on three components: polyethylene glycol, tris(hydroxymethyl)aminomethane, and sodium dodecyl sulfate (SDS). NGSCE showed a good linear relationship with satisfactory reproducibility between the migration time and the MW of standard proteins. It was found that the regenerated silk fibroin had an MW around 83 kDa with a wide MW distribution (MWD). This absolute value is lower than the result obtained from SDS-polyacrylamide gel electrophoresis due to the different principles of the methods, but their similar MWD shapes indicated that NGSCE could be a feasible, highly sensitive, rapid method for determination of the MW of silk fibroin. |
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